FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1582600
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24520.15493491722481992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC28150.040.420422
GTGGTAT29050.039.9376451
GTATCAA106550.025.4413171
GGTATCA56600.023.6067371
AGAGTAC77750.023.45276311
CAGAGTA79100.023.26037810
TATCAAC119100.022.7994332
TCAACGC127350.021.2354414
ATCAACG127850.021.1953873
AACGCAG129600.021.017876
CAACGCA129200.020.9526465
ACGCAGA144150.018.9363297
CGCAGAG143900.018.9155068
GTCGCTC1051.3057227E-418.33594928
GCAGAGT149650.018.1921679
AGTACGG100850.017.83539613
GAGTACG101900.017.78656612
CGGGATC4950.017.77862517
CGGGTCG1554.4857188E-717.7332526
GCTTGTA5700.016.8814331
Produced by FastQC (version 0.11.5)