FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923691
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21650.11254406242998485No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCA452.9275743E-547.221869
GTGGTAT12650.035.2811
GTATCAA41600.034.3311231
TGGTATC12800.033.5366442
GGTATCA25000.033.3240781
TGTACCG652.5529345E-432.694615
AGCGTCA2600.032.693763
CAACGCA45950.032.1894235
TATCAAC48550.031.1651332
ATCAACG48000.030.9909573
GCGTTAG550.004446083730.9128721
CGATCGC353.9457227E-430.35612150-51
TCAACGC50650.029.3702814
AACGCAG50400.029.178646
CTGTGCG3650.026.7806179
GTCGAGG8200.025.915118
AGTCGAG9150.025.5469177
ACGCAGA57350.025.418957
CGCAGAG58300.024.9318498
GCCGCAC1201.2766037E-524.792128
Produced by FastQC (version 0.11.5)