FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1923691
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45680.23746017421716897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG1200.034.37324524
GTGGTAT12700.032.7207031
CCGCGCG350.004102383331.42533528
TGGTATC13200.030.8381732
CGCGCGT453.7987824E-430.55240829
GCGACGA1650.029.99846623
ACGCTAG400.00784646127.520061
CGCGTAC550.001227592824.99742131
GTATCAA43200.024.4622761
CTAACGC701.800908E-423.5720633
ACGTGCG1052.1000233E-723.5714519
ACGGGCG701.8018605E-423.57022716
TAAGCCG2350.023.40610336
TACTAGG3800.023.1615312
CGAACTA600.00203724522.91430729
GGTATCA24100.022.0388871
TGCGACG1901.8189894E-1221.70941722
AGCCGTC2300.021.52343838
GAACCGT1155.056736E-721.5172846
TATCAAC49400.020.9901352
Produced by FastQC (version 0.11.5)