FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355065
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.15726182876836167No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAA19900.14685642386158598No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCT18200.13431090021511885No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCC17990.13276115905879055No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGA16100.11881348865183589No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC15450.11401667078701021No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAA15020.11084339127643322No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14490.1069321397866523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44500.044.9971161
GTGGTAT9200.043.8994981
TGGTATC9600.042.057932
TATCAAC50300.040.050312
TCAACGC51350.039.311234
CAACGCA52500.038.4501275
ATCAACG53000.038.1675763
GGTATCA21900.038.0483441
AACGCAG53550.037.8549236
ACGCAGA56600.035.815047
CGCAGAG58300.034.62498
GCAGAGT64700.031.2655569
CAGAGTA47750.025.89900610-11
AGAGTAC45750.022.66533910-11
CGTCAGA1703.289697E-722.4986825
GTACGGG54050.022.33027814-15
GAGTACG58550.021.01285712-13
AGTACGG49650.020.8849812-13
ACGGGAT10300.020.83692616-17
TACGGGG19250.020.53164514-15
Produced by FastQC (version 0.11.5)