FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1355065
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20970.15475272403906823No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT19380.14301896956972546No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC18210.13438469741303924No Hit
GTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGT15350.11327869880780628No Hit
GTACATTCCACAAGCATTGCCTTCTTATTTTACTTCTTTTAGCTGTTTAACTTTGTAAGAT14280.105382398630324No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGCTTGCTTCTCCT13860.10228291631766742No Hit
CCTCATTACGATTCGCCTGCTTGCTTCTCCTGTTCAATCGTTTCTTTGGAAGGCAGTGGAT13800.10184013313014505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42000.029.7462961
GTGGTAT9050.029.1911
TGGTATC9250.027.6550882
GGTATCA21150.026.8029861
TATCAAC47350.025.9669782
TCAACGC49000.025.0777634
CAACGCA49950.024.8191175
ATCAACG50200.024.590543
AACGCAG50600.024.5002986
ACGCAGA51800.023.8805377
AGAGTAC50850.023.6823411
CGCAGAG53150.023.2739738
CAGAGTA52850.023.14943710
CGGGATA1453.9108272E-1022.7580417
ACGGGAT9650.021.6574816
AGTACGG55950.021.37936413
GCAGAGT58350.021.2493239
CCGCTAG650.003232901721.161951
TACGGGG24050.020.35291315
CGGGATT3800.020.2626417
Produced by FastQC (version 0.11.5)