FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1611424
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32490.20162291240542526No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31610.19616190400540143No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.13205711221875804No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA16830.10444178565045575No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16280.10102865540044086No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20450.051.123542
GTGGTAT22400.047.0685731
GTATCAA76150.038.2426531
TATCAAC86150.033.7427062
GGTATCA43800.032.807261
TCAACGC89650.032.3779644
ATCAACG90600.032.1791843
CAACGCA92200.031.5746655
AACGCAG93450.031.1523176
CGCAGAG102950.028.319818
ACGCAGA103650.028.1686847
GCAGAGT111750.026.3178969
GTCGAGG11250.023.0446388
CAGAGTA70850.022.6155710-11
GACAGGT9000.022.2015191
GTCTAGG4850.021.0376341
GGTACGG2058.6693035E-820.731243
AGAGTAC68750.019.65895710-11
GAGTACG88000.018.11235212-13
AGTCGAG18100.018.0797027
Produced by FastQC (version 0.11.5)