FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457144
Sequences flagged as poor quality0
Sequence length91
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49520.33984287071147395No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35530.24383314209165327No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.17795084082286994No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT24250.16642143810083287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14700.051.1770862
GTGGTAT16150.046.063791
GTATCAA51050.039.4708751
TATCAAC56300.035.6330682
GGTATCA27900.034.587231
TCAACGC61100.032.759684
ATCAACG61300.032.6527983
CAACGCA61950.032.3101965
AACGCAG65650.030.3597456
CGCAGAG71200.028.2318
ACGCAGA71200.028.1713167
GCAGAGT77400.025.859799
GTCGTAC1452.074501E-623.4539571
CAGAGTA48050.023.43676210-11
AGAGTAC44950.021.3187610-11
GAGTACG53500.020.96984512-13
TAACGTG1254.7864113E-420.3986425
GTATAGG2952.7284841E-1120.1743771
GTACGGG57150.019.59338614-15
ACGTAGA1100.005472372819.3228631
Produced by FastQC (version 0.11.5)