FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542739
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17280.31838508012138433No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.25537136634736035No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10520.1938316575738983No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT7190.13247619942550654No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6690.12326366817199427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC7700.056.8466452
GTGGTAT8050.053.3536341
GTATCAA28500.038.0481761
TATCAAC31800.034.3450622
GGTATCA14900.033.106271
TCAACGC33500.032.602184
CAACGCA33750.032.3606835
ATCAACG33900.032.0921363
AACGCAG34550.031.4883736
ACGCAGA38300.028.4079287
CGACGGA600.00679868628.336569
CGCAGAG38950.027.9338558
CTAGACT1106.4915475E-627.043554
GCAGAGT40800.026.6697039
GACAGGT3700.026.4341051
TCGAGGT4350.026.3823139
GTCGAGG4400.026.0801128
GATTGAC850.001225565425.0005427
TAATACC2203.110472E-1023.1801854
CAGAGTA21750.023.15887510-11
Produced by FastQC (version 0.11.5)