FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005703
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52750.5245087267314505No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7100.034.8777961
TGGTATC8100.031.2295112
GGTATCA17500.028.929751
GTATCAA36350.028.3851851
TATCAAC41550.025.3448732
ATCAACG42400.024.3767833
TCAACGC42550.024.1568374
AGAGTAC40500.023.9664511
CAACGCA43600.023.954485
AACGCAG44500.023.3511246
CAGAGTA41850.023.19102910
ACGCAGA46150.022.4566847
CGCAGAG45950.022.4347748
ACGGGTA1757.2759576E-1222.0019116
GCAGAGT48100.021.4341059
AGTACGG44850.020.78775813
GAGTACG46350.020.34932112
AGTACGC1151.209453E-519.13304735
GTACGGG49600.018.6306514
CGGGATT3250.018.61717
Produced by FastQC (version 0.11.5)