FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786040
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15190.19324716299424965No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA11930.1517734466439367No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8740.11119027021525621No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT8720.11093583023764693No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAG8070.10266653096534528No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA7950.10113989109968959No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC7880.1002493511780571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGCG501.8247185E-532.985577
GTGGTAT18350.032.6705631
AGTACGC350.00409741331.43082413
TGGTATC18750.031.2303142
TTGTACG1101.2732926E-1129.9868833
ACCTCGC653.214931E-629.6024366
GAGCTAG400.00787133327.5002211
GCTTGTA3300.026.6668831
GTATCAA64650.026.2879141
CGGGCCG858.78199E-725.88420917
GCTTGTC1600.025.7814561
GGGCCGC651.08800305E-425.38643618
CTCGCGA651.0903583E-425.378368
CTTCGCC1351.6007107E-1024.446221
AGAGTAC48400.024.2615611
CAGAGTA49150.024.00171910
CGGGCCA701.799042E-423.57311817
CGCGTAG701.799042E-423.57311834
AACGCAG72000.023.3647796
TATCAAC72900.023.2301062
Produced by FastQC (version 0.11.5)