FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005413997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005413997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744029
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15730.21141649048625794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT13850.042.0863951
TGGTATC13750.041.9699672
GTATCAA47600.024.8957961
GGTATCA26750.022.8183821
TATCAAC53150.022.4392592
AGAGTAC32350.022.36058811
CAGAGTA32900.021.9031810
AGTGCGT650.003244550421.147258
CGGGATT1851.8189894E-1120.8127217
TCAACGC57650.020.4003354
ATCAACG57850.020.3311733
ACGGGAT7100.020.14404916
CAACGCA58850.020.0310485
CGGGTCA1108.238274E-620.00183517
TAGACCC700.00499697719.6274914
AGTACGG36250.019.57552713
AACGCAG60850.019.5546196
GTCCTAT1008.994878E-519.2465881
GAGTACG37350.019.14628412
TACGGGA19900.018.3823215
Produced by FastQC (version 0.11.5)