FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences914835
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11820.12920362688353637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17450.038.1218382
GTGGTAT18100.037.072781
GCTTGTA2200.030.0007741
GCGTAAT805.163947E-727.50823251
TTGTACG606.338826E-527.4856783
GTATCAA60550.025.3433461
TATCAAC67850.022.6067582
AGAGTAC45650.022.04876111
CAGAGTA46700.021.72968510
GGTATCA32950.021.3662591
ATCAACG72250.021.1516083
TCAACGC72250.021.1135654
AACGCAG73350.020.8332676
CTCTTAG1601.3369572E-920.6255341
CGGTGTA804.4421823E-420.62440545
CAACGCA74150.020.6096255
ACGCAGA81950.018.715087
CGCAGAG82400.018.4521458
TTCGGTA750.007433057318.3237863
CTTAGAC750.007433057318.3237863
Produced by FastQC (version 0.11.5)