FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1463267
Sequences flagged as poor quality0
Sequence length61
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG111870.7645221275406334No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10600.034.2667271
TGGTATC10450.034.212062
GTATCAA52200.029.0986751
TATCAAC58300.025.8973872
GGTATCA24150.025.7511081
AGAGTAC56400.024.61759611
TCAACGC61600.024.4999754
ATCAACG61850.024.4470623
CAACGCA62800.024.2498635
CAGAGTA58300.024.0502810
AACGCAG63250.023.992876
ACGCAGA66100.022.9999757
CGCAGAG66250.022.9064038
AGTACGG62150.021.94788413
GCAGAGT70400.021.6732489
GAGTACG65850.020.9609512
TACGGGG24750.020.55717815
GTACGGG69500.019.50873614
TCGACGT1654.2706233E-818.3347818
ACGGGAT10100.018.24401516
Produced by FastQC (version 0.11.5)