FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511136
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32060.21215827033437096No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27560.18237934904601572No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.1261302755013447No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.10402769836732101No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT20400.044.600311
TGGTATC20900.043.9227262
GTATCAA71700.039.3734321
TATCAAC79050.035.8596272
TCAACGC83800.033.8777284
ATCAACG84450.033.6169783
AACGCAG84600.033.4568986
GGTATCA40650.032.736941
CAACGCA86800.032.657885
ACGCAGA94900.029.9599957
CGCAGAG96900.029.4293428
GCAGAGT104000.027.5019539
CAGAGTA65850.023.39636810-11
GTCGAGG10800.022.430198
AGTCGAG16050.022.2426267
AGAGTAC62600.020.13012910-11
AGTACGG81000.019.0984542
GAGTACG83050.018.5832351
TCGAGGT11800.018.3683439
GACAGGT8350.017.8211561
Produced by FastQC (version 0.11.5)