FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511136
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22830.1510783940029223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT22700.039.627181
TGGTATC23650.037.5452582
GTATCAA84900.026.925551
TATCAAC93250.024.1740132
AGAGTAC66400.023.73245811
CAGAGTA68550.023.42942410
ATCAACG98400.022.8801123
TCAACGC98600.022.8615824
AACGCAG100100.022.4358736
CAACGCA100700.022.3567875
GGTATCA45100.020.8603131
CGGTCCG956.052519E-520.26693751
ACGCAGA111250.020.137167
CGCAGAG112300.019.9045338
GTATTAG5050.019.0654871
GCAGAGT118750.018.895999
AGTACGG85950.018.0783513
GAGTACG86900.017.97565812
TCGGTCA3000.017.4176115
GCTTGTC3900.016.9284761
Produced by FastQC (version 0.11.5)