FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688751
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA9370.13604335964666475No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT7410.10758605069175944No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24600.047.5132871
GGTATCA11100.042.8857041
TGGTATC6250.039.4397282
TATCAAC29750.038.5711672
GTGGTAT6550.038.284961
TCAACGC31100.037.3014034
ATCAACG31300.036.927293
CAACGCA31450.036.7538385
AACGCAG32200.035.897776
ACGCAGA33850.034.2734957
CGCAGAG34650.033.482198
GCAGAGT37250.031.1451789
GTATAGC808.604621E-426.5642471
TATACTG1457.0620445E-826.3772145
CAGAGTA30050.025.38483810-11
AGAGTAC28900.023.15992510-11
GACAGGT3400.022.501481
GAGTACG33650.022.47800812-13
ACGGGAT4300.022.23499116-17
ATAGCCG500.003158758421.2467716-17
Produced by FastQC (version 0.11.5)