FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076722
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51670.4798824580532394No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42960.3989887826198406No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.26107017410250744No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT20290.18844232773176364No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12720.11813634345727124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12950.053.4970132
GTGGTAT13050.052.766291
GTATCAA50000.040.3808671
TATCAAC56000.036.0512
GGTATCA27000.035.8941041
TCAACGC59450.033.9557344
CAACGCA60200.033.673895
ATCAACG60600.033.311363
AACGCAG63150.032.1008386
CGCAGAG68550.029.5707348
ACGCAGA70450.028.7732267
GCAGAGT73100.027.8464189
CAGAGTA41650.025.45697410-11
GACAGGT5850.025.4310441
GTACCGA1401.5325131E-624.2836047
GTCCATA1955.0968083E-821.7980381
AGAGTAC40300.021.51177810-11
GAGTACG49600.021.33383212-13
TACCGAC1708.256648E-619.9982648
GTACGGG53450.019.5983914-15
Produced by FastQC (version 0.11.5)