FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809053
Sequences flagged as poor quality0
Sequence length91
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33030.18258171540579518No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31170.17230009292154513No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20600.11387173289008115No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC22150.047.7743952
GTGGTAT23950.043.494491
GTATCAA75900.036.6921581
GGTATCA44150.033.995211
TATCAAC85700.032.7785872
GTCGAGG10850.030.5524778
ATCAACG92150.030.5303823
AACGCAG92850.030.4832956
TCAACGC92550.030.4443474
CAACGCA93200.030.277625
ACGCAGA103400.027.4141587
CGCAGAG104900.026.9823868
GCAGAGT113950.024.8774059
CAGAGTA68600.022.39637610-11
AGTCGAG18100.021.8360447
TCGAGGT15500.021.3873279
TATACCG1000.003157168421.2490525
AGAGTAC65700.019.9241110-11
GCTTGTA7150.019.0290721
AGTACGG84150.018.4335232
Produced by FastQC (version 0.11.5)