FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809053
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29460.16284763354086365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC24250.039.3275342
GTGGTAT26000.037.2326971
GTATCAA84100.025.8336811
AGAGTAC65750.023.05000911
CAGAGTA67150.022.73265810
TATCAAC95050.022.6406352
GGTATCA44500.021.8012663
ATCAACG100400.021.3241023
TCAACGC100950.021.1256644
CAACGCA101650.020.9801855
AACGCAG102050.020.9266176
ACGCAGA114450.018.660377
CGCAGAG116500.018.265278
AGTACGG83400.018.04049913
GAGTACG85500.017.75825312
GCTTGTA5000.017.600911
GCAGAGT122750.017.4063079
CGCGACA950.001408995517.37220424
TAACGGC1758.3686245E-817.28756536
GTCGAGG11650.016.7549638
Produced by FastQC (version 0.11.5)