FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600882
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15660.26061689316704445No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15050.25046514956347504No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.1674205584457514No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT8340.13879596992421142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10700.043.2942662
GTGGTAT10850.042.3145871
GTCGAGG4750.036.68418
GGTATCA20500.035.8747021
GTATCAA35100.035.728191
TATCAAC40350.031.1771092
TCGAGGT5550.029.8647799
TCAACGC42700.029.5608044
ATCAACG42800.029.4917353
AACGCAG43150.029.4495076
AGTCGAG7900.029.0505477
CAACGCA43700.028.981615
CGTCAGA1353.5264748E-828.333255
GACTCCG600.00680313628.3332487
ACGCAGA48800.026.3011537
CGCAGAG49000.026.1070658
ACGGGAG4950.025.75755
GGTACGG1001.0551201E-425.4999263
GCAGAGT52250.024.4831839
GCAGCGT1601.8239189E-723.912151
Produced by FastQC (version 0.11.5)