FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences600882
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7760.12914349239950607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11950.039.7918851
TGGTATC11900.039.47742
AGAGCGA400.00786053327.5069249
ACCTACG507.017654E-427.50691838
GTATCAA41750.026.2683451
AGAGTAC29900.024.19504711
CAGAGTA30750.023.88206310
TATCAAC46300.023.6157342
GGTATCA21150.023.3809153
ACTATCG952.3078683E-623.1637211
ATCAACG48450.022.5110683
TCAACGC48750.022.372544
AACGCAG49600.022.1553026
CAACGCA49800.021.9008275
GGGAGTC956.1000886E-520.242955
ATCGGTC1108.222572E-620.0050314
ACGCAGA55150.019.977197
GGTTAGC700.004966088619.64779723
AGGTCGG700.004968482519.6461610
CGCAGAG56000.019.6363438
Produced by FastQC (version 0.11.5)