FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1513022
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.17977266688785756No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25130.16609143819455366No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15770.10422849105961446No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12800.043.830362
GTATCAA49150.041.6889231
GTGGTAT13800.041.27841
GGTATCA24200.038.6459961
TATCAAC54750.037.1069152
TCAACGC55550.036.492394
ATCAACG56150.036.1024443
CAACGCA56500.035.87885
AACGCAG59450.034.1699266
CGCAGAG66200.030.6206258
ACGCAGA66750.030.4319847
GCAGAGT70650.028.8723899
TATACGG755.9081876E-428.3319685
GGTACCG1606.2536856E-926.5612186
CAGAGTA47400.024.74482310-11
CTATACG950.002344102822.3673424
AGAGTAC44850.021.5562210-11
GAGTACG53750.021.46569812-13
ACCGCGG2409.240466E-1021.2482749
TACCGCG2006.6820576E-821.2482748
Produced by FastQC (version 0.11.5)