FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029090
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.2858836447735378No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26370.25624580940442526No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.17500898852384145No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA17570.17073336637223177No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC16700.16227929529973084No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT16060.15606020853375313No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT15220.14789765715340739No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA14910.14488528700113693No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT14460.14051249161880885No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA14250.13847185377372243No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG13800.13409905839139435No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA13160.12787997162541664No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGC12790.12428456208883577No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC12770.12409021562739897No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA11630.11301246732550117No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.10834815225101789No Hit
CTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAA11080.10766793963598907No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGA10660.10358666394581621No Hit
GTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAG10550.10251775840791379No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17850.049.2855722
GTGGTAT20000.043.3667371
GTATCAA64300.040.2021371
TATCAAC73750.035.786342
GGTATCA36050.034.3197631
TCAACGC79100.033.312174
ATCAACG79350.033.2072143
AACGCAG81100.032.698696
GTCGAGG8500.031.9983568
CAACGCA82400.031.9780645
CGCAGAG90700.029.2846038
ACGCAGA91300.029.1386997
GCAGAGT97500.027.2857769
CGGGTCC1159.173404E-625.8694934
GGCACCG1551.3478166E-724.6761519
TCGAGGT11600.023.8134299
CAGAGTA61900.023.75541510-11
GCTTGTA4850.022.7923011
GTATAAC1805.6763383E-721.2582041
GCTAGCG500.00315699521.24942410-11
Produced by FastQC (version 0.11.5)