FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1029090
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT16160.15703194084093713No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16000.1554771691494427No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT15280.14848069653771778No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA14410.14002662546521683No Hit
GTATCAACGCAGAGTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCA13930.13536231039073357No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC13710.13322449931492872No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCT12640.12282696362805974No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCC11720.11388702640196678No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATAAGCTTCGGGGA11690.11359550670981158No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGATATTTGCCGCCT11520.11194356178759876No Hit
GTACGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAG11230.1091255380967651No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCCTTAGGAAAAGA10810.10504426240659223No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAA10680.103781010407253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21850.039.882252
GTGGTAT22150.038.746031
GTATCAA81950.026.3827421
AGAGTAC66000.023.62543711
CAGAGTA66100.023.54809210
TATCAAC92700.023.3381732
CGATCGT1202.9927833E-822.9215547
GGTATCA43400.022.7536491
ATCAACG98050.022.0076053
GCTTGTA4250.022.0057581
CCGATCG1254.616595E-822.00254646
TCAACGC98300.021.9516354
AACGCAG99700.021.8088156
CAACGCA101000.021.3909875
ACGCAGA109750.019.8136717
CGCAGAG110150.019.670678
GGACCTA700.004975150319.64322313
CTAGACT700.00498775419.634634
ATGCGGC856.69774E-419.4055217
CGGGACC2005.2750693E-1119.25035717
Produced by FastQC (version 0.11.5)