FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673057
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT120771.7943502556247093No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39660.5892517275654217No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32530.48331716333089175No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT23700.3521247086056605Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTCT21040.3126035387790336Illumina PCR Primer Index 10 (95% over 23bp)
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19850.2949230154355426No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC9130.135649729517708No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.11915781278554417No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7140.10608314006094581No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC9150.055.738532
GTGGTAT9750.054.0641371
GTATCAA35950.040.0860251
GTACGAC450.001663649237.7727283
TATCAAC41350.035.56282
GGTATCA19750.034.438571
ATCAACG44000.033.415993
TCAACGC44250.033.2271924
CAACGCA45450.032.4434055
AACGCAG46600.031.8251446
ACGCAGA52750.028.1147237
CGCAGAG52800.028.0076188
GCAGAGT56550.026.1503499
CAGAGTA28650.025.2889410-11
GACAGGT3800.023.4924281
AGACCGT1102.0165346E-423.1787196
GATCGAC2700.022.82441320-21
GAGTACG33200.021.75913612-13
CCTATAA1404.1904128E-521.2550531
AGAGTAC28250.021.1343410-11
Produced by FastQC (version 0.11.5)