FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673057
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220793.280405671436446No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC6050.037.2830052
GTGGTAT6300.036.6958661
CGCGTAG350.004097379731.43022734
ACGCGTA350.004097379731.43022733
TCACGCG350.004098870331.42789531
CACGCGT350.004098870331.42789532
GTATCAA33450.029.9490951
GACAGGT1600.027.5219021
TATCAAC37950.027.1814062
GGTATCA14600.025.2598291
ATCAACG39800.025.2193053
TCAACGC39750.025.0397624
AGAGTAC36300.024.99388711
CAACGCA40400.024.6332345
CAGAGTA37200.024.38919810
AACGCAG41100.024.213696
ACGCAGA42250.023.489557
CGCAGAG41200.023.4876568
GTACGAC600.002034914222.916173
GCAGAGT43600.022.1964059
Produced by FastQC (version 0.11.5)