FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2144179
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41910.19545942759443125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29750.040.569591
TGGTATC29800.039.5669362
GTATCAA122150.028.65221
AGAGTAC83750.026.10824411
TATCAAC134200.026.0306132
CAGAGTA87500.025.24019410
GGTATCA57250.024.587681
ATCAACG144850.023.9264243
TCAACGC144550.023.9184844
CAACGCA147550.023.5253035
AACGCAG150100.023.1638816
AGTACGG96150.022.54095513
GAGTACG100050.021.77225512
TCGACAC3300.020.8363422
ACGCAGA166850.020.8234547
CGCAGAG167950.020.6740788
GATCGAC3350.020.51960220
GCAGAGT173950.019.9819059
GTACGGG108200.019.7764214
GGATCGA5900.019.57407219
Produced by FastQC (version 0.11.5)