FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294615
Sequences flagged as poor quality0
Sequence length91
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35200.271895505613638No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34660.2677243813797925No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22240.17178852400134403No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT19860.15340468015587644No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA13730.10605469579759233No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.1036601615151995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC21700.046.4138872
GTGGTAT22050.046.087541
GTATCAA69950.037.8699341
TTGTACG2200.034.7703553
TATCAAC79200.033.7508542
TCAACGC83350.031.9684264
ATCAACG84050.031.7527433
AACGCAG84950.031.3162886
GGTATCA41150.030.9988541
CAACGCA86150.030.8800755
GCTTGTA7300.027.9584181
ACGCAGA95850.027.7993567
CGCAGAG96750.027.5846848
GTCGAGG13350.026.739758
GCAGAGT105600.025.5153479
TCGAGGT13650.024.907699
CAGAGTA59400.022.35834510-11
AAGTACG1955.081165E-821.8051781
AGTCGAG20600.020.8359587
AGTACGG73000.020.4335382
Produced by FastQC (version 0.11.5)