FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414039

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414039
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294615
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19430.1500832293770735No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT13080.10103389810870414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25950.037.498222
GTGGTAT26650.037.0418781
CGATCGT852.0008883E-1135.59621447
CGACGGT400.0078767627.49766319
GTATCAA90400.025.9159371
CGGTCCG550.001227246524.9978759
AGAGTAC63750.024.54775411
CAGAGTA65550.024.04150410
TATCAAC97250.023.827732
GATCGTA1302.741217E-923.27534948
TCAACGC104400.022.1678094
ATCAACG104250.022.1469753
AACGCAG105000.022.017516
CAACGCA106750.021.7055535
GCTTGTA5850.021.1520461
GGTATCA47900.020.8870473
ATCGTAG1902.5465852E-1120.26848449
ACGCAGA116950.019.7920237
CGCAGAG117000.019.7850938
AGTCCGG1552.082379E-819.51975354
Produced by FastQC (version 0.11.5)