FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752675
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT26970.15387907056356712No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19550.11154378307444335No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT19310.1101744476300512No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGTTCGCTCGCCGC19240.10977505812543684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC12450.032.6997532
GTGGTAT12850.031.490271
GTATCAA66900.030.983541
GGTATCA30050.028.489061
TATCAAC77050.027.1327042
ATCAACG79550.026.1680583
TCAACGC79500.026.1093754
CAACGCA80850.025.8086935
AACGCAG81050.025.7125556
AGAGTAC78300.025.42540211
CAGAGTA79650.024.92328510
ACGCAGA84200.024.7832787
CGCAGAG85350.024.5144738
GCAGAGT90000.023.3096569
AGTACGG88750.021.9700413
GAGTACG90500.021.81683212
ACGCCGT700.0049732519.64545634
CCGACCG700.00498064319.64041125
GTACGGG101350.019.02160814
ACGGGAA9550.019.00612316
Produced by FastQC (version 0.11.5)