FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1287115
Sequences flagged as poor quality0
Sequence length61
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19770.15359932873130994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23000.038.263091
TGGTATC23200.037.4445082
GTATCAA82200.025.694921
TATCAAC90250.023.2111592
AGAGTAC63650.022.98640611
GGTATCA45050.022.7094171
CAGAGTA64950.022.69569610
ATCAACG97600.021.5170143
TCAACGC98000.021.3450434
AACGCAG99400.021.1566666
GCTTGTA4550.021.1550731
CATACGA650.00324544621.14767331
CAACGCA100100.020.8972455
CGACTAT804.4455953E-420.6229919
ACGCAGA111200.018.9363467
CGCAGAG112550.018.6618128
AGTACGG80500.018.03901513
GTCGAGG11750.018.0160188
GCAGAGT117150.017.9339189
GAGTACG81150.017.86063412
Produced by FastQC (version 0.11.5)