FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences825074
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14750.17877184349525013No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA12240.14835032978860077No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT9070.10992953359335042No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATCCGGAGATTTCT8790.10653589859818634No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCCTA300.001924141136.67119612
TGGTATC19500.035.3867872
GTGGTAT20100.034.3533671
GACAGGT2500.028.610471
GTATCAA71200.025.5395431
AGAGTAC52200.025.3416311
CAGAGTA52400.025.2973910
GCTTCCT1101.1854354E-825.0091511
GCTTGTA3750.024.9424631
TATCAAC78200.023.2730462
AACGCAG80300.022.6972716
ATCAACG82250.022.1243953
CGCGGCC1505.966285E-1022.0013811
TCAACGC82500.021.9907154
CTTGAGT1901.8189894E-1221.7039932
CAACGCA84150.021.690195
ACGGGCT3450.021.52439716
CGGGCTC903.9900668E-521.3915317
TGTCCCG903.991933E-521.39023211
CGCCGTA650.003237804221.15517625
Produced by FastQC (version 0.11.5)