FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702706
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32220.4585132331302138No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30000.4269210736780389No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19030.270810267736436No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.22541432690200455No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13030.18542605300082823No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.18485682490259084No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAA9970.14188010348566826No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC8550.12167250599824109No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA8380.1192532865807322No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC8330.11854175145793547No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA7670.10914948783701862No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT7530.10715718949318777No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7360.10473797007567887No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC7180.10217644363361064No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7100.10103798743713588No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC25800.036.2383652
GTATCAA75350.035.5423851
GTGGTAT27400.033.5113951
GACAGGT2350.032.5607151
AACGCAG84650.032.33146
TATCAAC87400.031.1682362
AAGTACG1659.094947E-1230.9162371
GCTTGTA3450.029.572051
ATCAACG92500.029.5416583
TCAACGC93000.029.5199224
CAACGCA94250.029.2185965
ACGCAGA95050.028.838547
CGCAGAG96350.028.4955718
GACAGAT1652.7830538E-1028.3398841
ACAGGTA2950.027.3792081
GCAGAGT100900.027.2105889
ATACGGG2700.026.7654441
GGTATCA45000.026.0726911
GTCGAGG10050.024.5277948
AGCGTCA2152.3283064E-1023.719663
Produced by FastQC (version 0.11.5)