FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences702706
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11660.1659299906361978No Hit
GTACGGGACTATCACCCTCTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAA9570.13618782250329442No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCT7430.1057341192475943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG358.694593E-539.2624328
GGCTAGC300.001927354836.65797819
GCTTGTA2900.035.082891
GGACAGT501.8272762E-532.9780927
TGGTATC27050.032.4137652
GTGGTAT27750.031.5105361
GCGAGTG350.004093311731.43679439
CGACGTA400.0078774527.4954439
CCGGGAC1156.6029315E-1026.3112322
CTTGTAT2300.026.2906282
CTGCTTA2450.024.70033818
GTATCAA84050.024.3403531
GATACCA2650.023.8707711
CGCGCAG701.796462E-423.57759749
CGAGGAC1052.090801E-723.57759728
CAGAGTA56650.023.54481910
AGAGTAC56200.023.53928411
GCTTGGC952.3157518E-623.1557041
TGCTTAA2750.022.9945519
GGGCCTC600.002031653222.9226638
Produced by FastQC (version 0.11.5)