FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968786
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45500.46965996618448247No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30860.3185430012407281No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20400.21057282000359212No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT15550.160510164267444No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA9750.10064142132524623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC13250.054.2086682
GTGGTAT13000.053.9601361
GTATCAA45150.038.700491
TATCAAC51700.034.115792
CGGGTCG500.00278919533.9989476
GGTATCA25900.033.9784051
TCAACGC54200.032.6973274
ATCAACG54300.032.480583
CAACGCA54900.032.3578345
AACGCAG57100.031.111126
CGCAGAG63200.028.1068558
ACGCAGA65050.027.3075057
GCAGAGT66800.026.5921159
CAGAGTA39950.024.94474610-11
AGAGTAC38300.022.80164110-11
GTACAAT1903.862806E-822.3758131
GTCTAAG1353.164955E-522.044321
GAGTACG45150.021.78945212-13
GACAGGT4500.021.72941
GTACCTA1203.6228518E-421.257021
Produced by FastQC (version 0.11.5)