FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968786
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34630.3574576841531566No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6650.034.348071
TGGTATC7150.031.923022
GTATCAA39600.031.2030531
GCTTGTA1900.028.968251
GGTATCA17250.028.237751
TATCAAC45950.027.110942
TCAACGC48250.025.6966554
CAACGCA48300.025.671385
CAGAGTA46000.025.58558710
AGAGTAC45400.025.56030711
ATCAACG48700.025.5156633
AACGCAG49950.024.8784166
CGCAGAG50750.024.2166298
ACGCAGA52600.023.6773037
GCAGAGT53600.022.9838479
AGTACGG50950.022.34654813
GAGTACG52500.021.84281712
TACGGGG18650.020.64445915
CCGAAAT804.4385422E-420.6270952
ACGGGCT2850.020.26416616
Produced by FastQC (version 0.11.5)