FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051272
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40230.38267926854325046No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT31940.30382241703384094No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.22496556552443134No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT16290.15495514005890007No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.14116232525930492No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT11530.10967665837195323No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16700.056.4929282
GTGGTAT17950.052.568881
GTATCAA63700.039.769221
GGTATCA33850.037.4194831
TATCAAC72100.035.306052
TCAACGC77050.033.258474
ATCAACG77750.032.9590343
CAACGCA78750.032.540515
AACGCAG80400.031.714136
GTCGAGG10850.030.9424698
GGACCGT1003.0958163E-629.7478526
CGCAGAG89750.028.5049068
AGCGTCA1503.1395757E-928.331293
ACGCAGA90800.028.2220827
TCGAGGT12450.026.9659259
GCAGAGT95300.026.934059
GCGTCAG1606.2500476E-926.5605834
CGTCAGA1751.621811E-824.2839625
CAGAGTA50100.023.92156610-11
AGTCGAG17600.023.9045267
Produced by FastQC (version 0.11.5)