FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051272
Sequences flagged as poor quality0
Sequence length61
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30370.2888881279060034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20950.044.2127842
GTGGTAT21750.043.3592221
GTATCAA73800.026.6004181
GGTATCA38200.024.6792963
AGAGTAC47150.024.55675711
TATCAAC81400.024.0750452
CAGAGTA49350.023.73954610
GACAGGT4350.022.7541121
ATCAACG88200.022.000783
TCAACGC88100.021.9623154
CCTATAC1155.0680137E-721.510313
CAACGCA90200.021.4814665
AGTACGG54350.021.35522813
AACGCAG91650.021.142616
GAGTACG56250.020.53610612
TACGGGC7450.020.30475415
TACGGGG22150.019.4947315
CGGGGGA3350.018.88224617
GTACGGG61150.018.84555414
ACGCAGA105050.018.5006647
Produced by FastQC (version 0.11.5)