FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594927
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63020.39512780208749365No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47780.29957483947541175No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34680.2174394188574148No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT25460.15963113045299251No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23800.1492231305884219No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.10382920346824652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT23400.061.591841
TGGTATC24600.059.256362
GTATCAA92200.038.733591
TATCAAC101900.035.4920732
GGTATCA48600.034.991541
TCAACGC108250.033.4100884
ATCAACG108250.033.370833
CAACGCA110850.032.6647875
AACGCAG115650.031.4560436
GTCGAGG17250.028.5797548
CGCAGAG128750.028.2563518
ACGCAGA129700.028.015747
GCAGAGT133350.027.3152149
TCGAGGT18550.026.3494019
CAGAGTA67700.025.11239410-11
AGTCGAG26600.022.3677547
ACGGGAT14550.022.05260716-17
AGAGTAC66050.021.97529410-11
GAGTACG79150.021.37218512-13
TATACCG1050.004188078520.237494
Produced by FastQC (version 0.11.5)