FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1594927
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43470.2725516591041471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT29300.043.64271
TGGTATC29950.042.2165682
GTATCAA117800.027.2661481
AGAGTAC68250.025.38742411
TATCAAC126900.025.1473672
CAGAGTA70750.024.6442910
ATCAACG134950.023.5010223
GGTATCA57750.023.1280023
TCAACGC136700.023.0996484
CAACGCA138650.022.8342345
AACGCAG141500.022.3354766
TACGGGG34200.021.87376415
AGTACGG79500.021.8294613
GAGTACG81100.021.33096712
ACGGGGT10400.019.83454916
ACGCAGA159800.019.813927
CGCAGAG160500.019.7147068
CCGATCC700.00497367719.64503136
CGTATAA700.004982709419.6388730
GTACGGG88250.019.50924114
Produced by FastQC (version 0.11.5)