FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161520
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT62840.5410152214339831No Hit
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48290.4157483297747779No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33770.2907397203664164No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT25450.21910944279909086No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.11140574419725875No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC16000.053.6620942
GTGGTAT16250.053.6396981
GTATCAA60100.038.345171
GGTATCA32750.038.299811
TATCAAC67550.034.2302252
TCAACGC70750.032.6177144
ATCAACG70850.032.571683
CAACGCA71600.032.289855
AACGCAG73650.031.5064896
CGTACGA853.4934095E-529.996819
CGCAGAG82050.028.330328
ACGCAGA82550.028.2102037
GCAGAGT86050.026.9640129
CAGAGTA47500.025.5420210-11
GAGTACG53150.022.82732212-13
GACCCGC1353.1742355E-522.036596
AGAGTAC46000.021.66345810-11
GTACGGA1404.1868152E-521.2596381
GTACGGG57900.020.88166414-15
ACCCGCG1050.004189258420.2359437
Produced by FastQC (version 0.11.5)