FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161520
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57920.4986569322956127No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC10400.036.747732
GTGGTAT10750.036.0876851
GTATCAA47600.027.9760741
GGTATCA23350.027.101251
TATCAAC54250.024.5805192
TCAACGC57800.022.8726624
ATCAACG57700.022.8666363
AGAGTAC51850.022.69767811
CAACGCA58800.022.4846385
AACGCAG59450.022.2416746
CAGAGTA53800.021.97437510
CGCAGAG61650.021.4043058
ACGCAGA62800.021.0552147
GACGGTA804.4571457E-420.6148135
GCAGAGT64300.020.6076769
AGTACGG57850.019.9200613
TGCCGAC5700.019.78370955
GAGTACG59100.019.7280212
ACGGGAT12850.019.04876716
TACGGGG24250.018.82679215
Produced by FastQC (version 0.11.5)