FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630670
Sequences flagged as poor quality0
Sequence length61
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9380.14873071495393786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT10450.042.122091
TGGTATC11400.038.5843622
CTATCGG504.120502E-738.5001112
ACTATCG458.83768E-636.6667711
TAACGGC300.001924937136.6667736
ATCGGTC553.4882996E-530.00008614
TATCGGT653.2026E-629.61546913
ACGCGTA400.0078734227.49789633
CACGCGT507.034775E-427.49571832
CTATACC759.767116E-625.65864
GTATCAA36550.025.1400031
AGAGTAC23100.023.57149711
CAGAGTA24100.022.9357510
TATCAAC40200.022.8410782
GGTATCA20450.022.4501533
CCGTTAT752.8629182E-422.0087931
ATCAACG43150.021.3432333
TCAACGC43100.021.2404214
ACCCGTT650.00323660521.15558441
CAACGCA43850.020.8771325
Produced by FastQC (version 0.11.5)