FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408868
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44280.3142948807127424No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT33610.2385603193485834No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.18120931130524648No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18910.1342212329331066No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT18770.13322752734819726No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC14900.055.620462
GTGGTAT17050.051.3760031
GTATCAA74650.044.316681
TATCAAC82450.040.102842
TCAACGC85050.038.7269744
ATCAACG85300.038.6632963
CAACGCA86700.037.940945
AACGCAG90200.036.4674346
GGTATCA35000.036.4477461
CGCAGAG97950.033.496478
ACGCAGA98800.033.2931447
GCAGAGT105950.030.8870169
GTCGAGG10550.029.0045598
CAGAGTA61000.025.18721210-11
TGGACCG850.001226737224.9998445
AGAGTAC58400.022.9608610-11
TCGAGGT12700.022.7557629
AGTCGAG18900.021.811267
GAGTACG71750.021.2654312-13
AGTACGG68050.019.4862412-13
Produced by FastQC (version 0.11.5)