FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408868
Sequences flagged as poor quality0
Sequence length61
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24500.17389847735912803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC20850.043.370942
GTGGTAT22750.040.0031661
GTATCAA89100.027.2786141
AGAGTAC60950.025.22282811
TATCAAC96400.024.9482462
CGGGATA4550.024.7815317
GGTATCA44100.024.5020081
CAGAGTA63500.024.25325210
ATCAACG104050.023.0603433
TCAACGC103900.023.0407264
GGGCGTC600.002041440522.9056787
CAACGCA106300.022.6756575
ACGGGAT15150.022.32791316
AACGCAG108900.022.2360176
AGTACGG71350.021.39216213
GAGTACG73750.020.9197512
CGGGATC5800.020.86317417
ACGGGGC4550.020.55053716
ACGCAGA121750.019.9124187
CGCAGAG122100.019.8602738
Produced by FastQC (version 0.11.5)