FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139011
Sequences flagged as poor quality0
Sequence length91
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14561.047399126687816No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10290.7402291904957161No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.6236916503010553No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5650.4064426556171814No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.3992489802965233No Hit
ACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5210.37479048420628586No Hit
GTGGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.29494068814698116No Hit
GGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3380.24314622583824302No Hit
ACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.22012646481213718No Hit
GTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.22012646481213718No Hit
ATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.2136521570235449No Hit
TATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2630.18919366093330744No Hit
GGTATCAACGCAGAGTCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2140.15394465186208287No Hit
ACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1990.14315413888109574No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.14171540381696413No Hit
GAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1860.13380236096424022No Hit
TCATAGGACAGAGGAGTGAGTTCTGTCAACAGACAGGCGGCCCCTACTTG1800.12948615577184538No Hit
GTGGTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.12876678823977958No Hit
GTATCAACGCAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1670.1201343778549899No Hit
CTCCTGGGCCGCGTGAAGGCCTTCGATAGGCACTGCAACATGGTGCTGGA1540.1107825999381344No Hit
GAACAACACTGTGCTCTTGTAGGTTATGGCCTTCACCACCGATATAGCTA1410.1014308220212789No Hit
GTACTAAACGTCCTAAGAAATAATCTTTTAAAGATTAGCTTCTTAAGAAT1410.1014308220212789No Hit
GGTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.10071145448921309No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGCT203.0196923E-584.997129
ATTAGGC203.0196923E-584.997128
ATATTAG203.0196923E-584.997126
CATTATA200.003217953363.7478452
GTGGTAT4750.063.5470241
TGGTATC4850.062.214392
CAGTGGT551.7479779E-646.3787541
AGTGGTA551.7523198E-646.362072
GCTATTT209.292274E-442.51385512-13
TAGGCTA209.292274E-442.51385510-11
GGCTATT209.292274E-442.51385512-13
ATATCAA701.8781793E-742.513851
TGTGCAT409.292657E-442.498569
CTTATAT409.292657E-442.498563
GGTATCA9600.037.6424751
GTATCAA16700.036.9132271
ATATAAC500.002781063633.9988489
TATCAAC19700.033.2222252
TAAACGC402.3014867E-531.87392222-23
TTAAACG402.3014867E-531.87392222-23
Produced by FastQC (version 0.11.5)