FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836165
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21420.2561695359169542No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19000.22722787966489869No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC12870.15391699006774978No Hit
GGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12300.14710015367780283No Hit
GTGGTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTT11220.13418404262316647No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA10470.1252145210574468No Hit
GTATCAACGCAGAGTCGACGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.107993039651265No Hit
GTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTTCTCTTGTCCTTTC8810.10536197999198724No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT8770.10488360550848218No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC17450.039.9401052
GTATCAA56950.037.7677841
GTGGTAT18600.037.251121
AACGCAG63450.034.4285136
TCAACGC67450.032.3218544
TATCAAC68950.031.7419642
CAACGCA69050.031.636345
ACGCAGA69500.031.43157
ATCAACG69650.031.3619333
CGCAGAG69800.031.2964068
GCAGAGT75050.029.1637469
GGTATCA32150.028.823071
GGCACTA600.006804700528.3331956
GACAGGT4150.026.6311531
GGCACCG5750.026.6085649
GTCTTAC808.600707E-426.5671371
GTCGAGG7500.025.4998748
GTATTAG9250.024.8151421
CAGAGTA47500.024.69461410-11
AGACAGG4650.023.7675881
Produced by FastQC (version 0.11.5)