FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385979
Sequences flagged as poor quality0
Sequence length91
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA7730.20026996287362786No Hit
ACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCACAGGAT5810.15052632397099325No Hit
ACTATGTATTCATCATGCGATACCTACCTTATGGTAAGTGGGTTTCCCCA5600.14508561346601756No Hit
ACGTAATAGCCTGCGATAAGCTTCGGGGAGGCGGCAAATATCCTGTGATC5330.13809041424533458No Hit
ATACATAGTCATGCGAGGCGAACGAGGGGAAGTGAAACATCTCAGTACCC5030.13031797066679793No Hit
TCCCAGAGCTTTCCGCTAAAACACATATAACTTAATGGGCTGATCCCCGT4820.12487726016182227No Hit
GGGTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTAT4410.11425492060448884No Hit
CCATAAGGTAGGTATCGCATGATGAATACATAGTCATGCGAGGCGAACGA4280.1108868617204563No Hit
GTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.10829604719427742No Hit
GTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTAATAGCCTGCGATA4080.10570523266809852No Hit
CTACGGTAGCACTTCCCAGAGCTTTCCGCTAAAACACATATAACTTAATG4010.1038916624997733No Hit
ATACCTACCTTATGGTAAGTGGGTTTCCCCATTCAGAAATCTCCGGATCA3950.10233717378406598No Hit
GTACTGAGATGTTTCACTTCCCCTCGTTCGCCTCGCATGACTATGTATTC3860.10000544071050498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTATC3450.044.337352
GGTATCA7400.044.246921
GTATCAA14400.042.5230141
GTGGTAT3700.040.2244721
TATCAAC16550.037.2268522
ATCAACG16850.036.3118933
TCAACGC16850.036.3118934
CAACGCA17450.035.0633475
AACGCAG17550.034.863566
ACGCAGA18700.032.7237787
CGCAGAG19450.031.4619378
GCAGAGT20750.029.4908289
CGTCAGA755.906643E-428.3266375
TCAGATG808.606401E-426.5596667
GTACTGG1457.002927E-826.3935951
GCGTCAG850.001226767324.9940934
CAGAGTA17500.024.52595110-11
AGCGTCA900.001711365523.6055343
AGAGTCG3550.022.7411045
GTACCTA950.002332958922.3805331
Produced by FastQC (version 0.11.5)