FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1162670
Sequences flagged as poor quality0
Sequence length61
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84990.7309898767492066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7450.035.083581
TGGTATC7600.034.0115622
GTATCAA41450.029.8028891
TACCGTC705.716731E-627.4939797
TATCAAC46400.026.84682
GGTATCA19350.026.446521
CAACGCA49900.024.7931025
TCAACGC49350.024.7908694
AGAGTAC44100.024.62930111
CAGAGTA45600.024.60199410
ATCAACG49850.024.5973663
AACGCAG51350.023.9869586
CGCAGAG52450.023.3790558
ACGCAGA53000.023.1364427
GCAGAGT54600.022.4603849
AGTACGG47650.022.16246213
TACGGGG19200.021.9149215
GAGTACG49850.021.34804312
GTACGGG52750.019.86333714
ACGGGGG6100.018.4843316
Produced by FastQC (version 0.11.5)