FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005414079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005414079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333869
Sequences flagged as poor quality0
Sequence length61
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25210.18899906962377863No Hit
GTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCT15910.11927708043293606No Hit
GACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGCAACCATGTCTGAC14680.11005578508834077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT16600.040.7455371
TGGTATC16500.039.9776652
GTATCAA66550.028.2592771
AGAGTAC45200.026.34611311
TATCAAC73500.025.2036082
CAGAGTA48550.024.64149710
GGTATCA31350.023.9429761
TCAACGC76600.023.8597974
ATCAACG77200.023.7811583
CAACGCA78300.023.3768655
AACGCAG78600.023.2552916
AGTACGG53100.022.271913
GAGTACG56050.021.19703912
ACGCAGA89750.020.336337
CGCAGAG90900.020.055588
TACGGGG24450.019.910315
GCAGAGT95500.019.1478569
GTACGGG62100.018.82264514
CGGGGTT3650.018.08362217
TACGGGA29800.017.4433115
Produced by FastQC (version 0.11.5)